I.A. Koltakov, O.S. Ivanova, E.V. Zinkova, V.G. Artyukhov

In the last few decades, the practices of medicine have been used inductors thesis of endogenous interferon. However, the lack of information on the mechanisms of their action on the cells of the immune system causes number of difficulties in selecting he most effective concentrations for each patient, control reactions to cytokines produced by immune cells in response to their introduction and predicting the outcome of treatment. We have studied the mechanisms of action of cycloferon - one of the high synthesis of endogenous interferon inducers, the manifestation off unctional activity of T-lymphocytes in human blood, measured by the change in the level of one of the key componentsof T-cellantigenreceptor - CD3 sets. It was found that the number of CD3 epitopeson the surface membrane of T cellsisa complexfunction of the concentrationcycloferon and-interferon synthesizedby cellsin response to itsintroduction. The use of protein synthesis blocker actinomycin-D and cycloheximiderevealed the immunosuppres sive effect of the studied immunomodulatoron T-lymphocytesin human blood. To elucidate them echanisms of the effect of cycloferonon the structure membranes,we studied the expression level of surface differentiation antigens, a series of experiments to study the ability of the membranes of T-lymphocytesto bind the fluorescent probe 1,8-ANS. Reductions in the intensity off fluorescence of the probe in proportion to the concentration of the modifier were reported. Simultaneously was recorded fluorescence shift of the maximum from 472 nm to 483 nm. In order to eliminate the effect of modifying our study was reported separately cycloferon and fluorescence spectra of ANS in the membranes of T-lymphocytes. Analysis of the results showed that, despite the close proximity of the absorption band scycloferon and ANS studied drugs not make an error in the spectral characteristics of the probe ANS, and were corded decrease in intensity and shift of the maximum fluorescence of the probeisdue to the fact that by interacting with the membrane of lymphocytes, cycloferon block savailablebinding sites of the probe and, consequently, can make a significant contribution to the change in the local microenvironment of trans membrane proteins, some of which are components of the studied T-cell receptor for antigen, and will affect the functional activity of T-cell immunity. The level of expression of the studied receptor son the membranes of T-lymphocytes in the experimental conditions is complicated depending on the applicable modifiers. It is defined as the concentration of cycloferon and concentration of γ-interferon synthesized by T cellsin response to its introduction. In this regard, a significant opportunity for the regulation of T-cell immunity influences the synthesis of interferon inducers, and our data on the mechanisms of influence on the expression level of cycloferon CD3 complexes are of great importance in clinical practice to predict the expected effects during the treatment of this group of drugs in patients with different pathologies.