350 rub
Journal №11 for 2013 г.
Article in number:
Inducible NO-Synthase as enzymatic biotest-system for detection of substances with anti-inflammatory properties in vitro
Keywords: enzymatic biotest-system in vitro inducible NO-synthase (iNOS) anti-inflammatory properties biologically active substances (BAS)
Authors:
L.B. Strelkova - Ph. D. (Biol.), Heading Research Scientist, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (VILAR), Moscow, Russia. Е-mail: strelkova46@bk.ru
N.V. Kondakova - Dr. Sc. (Biol.), Heading Research Scientist, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (VILAR), Moscow, Russia. Е-mail: kondakova24@mail.ru
V.A. Dubinskaya - Ph. D. (Biol.), Heading of Department of Biomedical Technologies, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (VILAR), Moscow, Russia. Е-mail: va@diamsoft.ru
V.A. Bykov - Dr.Sc. (Eng.), Professor, Academician RAMS and RAAS, Adviser of RAAS. Е-mail: vilarnii@mail.ru
Abstract:
Тhe conditions for application of inducible NO-synthase (iNOS) as enzymatic biotest- system was investigated for identification of biologically active substances (BAS) with anti-inflammatory activity in vitro. The effect of BAS on the enzymatic activity of iNOS was determined by spectrophotometrically in a kinetic mode. For verification and approbation the biotest- system in vitro was used non-steroidal anti-inflammatory drags (NSAIDs) with the established anti-inflammatory properties - movalis, ketonal, voltaren and the substances relating to other pharmacological groups. The ingibitory effect of NSAIDs on the enzymatic activity of iNOS in vitro is revealed. It was defined that movalis has the strongest ingibition on iNOS-reaction compared with ketonal or voltaren. The anti-inflammatory properties was found in the activity of antibacterial drags - metronidazole, nitazole and antidiabetic agent metformin. Use the new specific enzymatic biotest- system on the basis of iNOS in vitro will allow to select BAS with anti-inflammatory activity for carrying of preclinical tests.
Pages: 75-80
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