E.A. Sizova, S.A. Miroshnikov, S.V. Lebedev, A.V. Skalny, N.N. Glushenko

At present the use of vital metals in form of nanoparticles being a source of elements for the organism and potential drug carrier is prospective. The timeliness may be confirmed by the absence of common opinion on safety of copper nanoparticles administration to organism. The research of the most common regularities of reflected biological and toxic effects of nanoparticles depending on their form, size, source material, surface area, charge and other physical and chemical structural peculiarities as well as doses, ways of administration, concentration in target organs and exposure time are the most important questions in toxicology of nanoparticles and need detailed study. Mineral composition of rat tissues has been researched for 25 elements by means of atomic emission and mass spectrometry at one-fold and manifold intramuscular administrations of copper nanoparticles sized 103,0 ± 2,0 nm and covered by oxide film of 6 nm and phase composition of crystalline copper 96 %, copper oxide 4 %. The conducted studies make us to assume that there is no critical change in common pool concentration of microelemenents, toxic and essential elements as well as in content of administered copper in tissues of animals at manifold administration of copper nanoparticles in biotic doses to organism. This attests to the absence of irregularities on the part of system of metals homeostatic regulation in organism at nanoparticles introduction. Though, histological examinations and studies of cell apoptosis confirm the presence of significant structural changes in tissues and progressive increase of apoptosis in cells up to the 12th week of metal administration. This attests to the fact that some or group of elements significantly influence on biological response to manifold administration while common pool of macro- and microelements is rather stable.

 

1. Cappuis P., Aral B., Ceballos-Picot I. Copper related diseases // Metal Ions in Biology and Medicine/ Eds Ph. Collery, P. Bratter, V. Negretti de Bratter, L. Khassanova, J.C. Etienne. Paris: John Libbey Eurotex. 1998. V. 5. P. 729-736.
2. Yang Z., Liu Z., Allaker R. et al. A review of nanoparticle functionality and toxicity on the central nervous system // J. R. Soc. Interface. 2010. Suppl. 4. S. 411-422.
3. Shvedova A.A, Kagan V.E. The role of nanotoxicology in realizing the \'helping without harm\' paradigm of nanomedicine: lessons from studies of pulmonary effects of single-walled carbon nanotubes // J. Intern. Med. 2010. V. 267(1). P. 106.
4. Chen Z., Meng H., Xing G. et al. Acute toxicological effects of copper nanoparticles in vivo // Toxicol. Lett. 2006. V. 163(2). P. 109.
5. Sharma H.S., Hussain S., Schlager J. et al. Influence of nanoparticles on blood-brain barrier permeability and brain edema formation in rats // Acta Neurochir Suppl.2010. V. 106. P. 359-364.
6. Wright J.B., Lam K., Buret A.G. et al. Early healing events in a porcine model of contaminated wounds: effects of nanocrystalline silver on matrix metalloproteinases, cell apoptosis, and healing // Wound Repair Regen. 2002. V. 10(3). P. 141.
7. Durnev A.D. Toksikologija nanochastic // Bjulleten ehksperimentalnojj biologii i mediciny. 2008. T. 145. № 3. S. 78-80.
8. Sizova E.A., KHolodilina T.N., Miroshnikov S.A., Poljakova V.S., Glushhenko N.N. K razrabotke kriteriev bezopasnosti nanochastic metallov pri vvedenii ikh v organizm zhivotnykh // Vestnik Rossijjskojj akademii selskokhozjajjstvennykh nauk. Moskva. 2011. № 1. S. 40-42.
9. Sizova E.A.Poljakova V.S., Glushhenko N.N. Morfofunkcionalnaja kharakteristika selezenki krys pri vnutrimyshechnom vvedenii nanochastic medi // Morfologija. 2010. T. 137. № 4. S. 173.
10. Vernikov V.M., Arkanova E.A., Gmoshinskijj, I.V., KHotimchenko S.A., Tutelen V.A.Nanotekhnologii v pishhevykh proizvodstvakh: perspektivy i problemy // Voprosy pitanija. T. 78. № 2. 2009. S. 4 - 17.
11. Glushhenko N.N., Bogoslovskaja O.A., Sizova E.A., Poljakova V.S., Miroshnikov S.A. Izuchenie bezopasnosti vvedenija nanochastic metallov v organizm // Materialy XVI Rossijjskogo nacionalnogo kongressa «CHelovek i lekarstvo». Moskva. 2009. S. 533.
12. Bogoslovskaja O.A., Sizova E.A., Poljakova V.S., Miroshnikov S.A., Lejjpunskijj I.O., Olkhovskaja I.P., Glushhenko N.N. Izuchenie bezopasnosti vvedenija nanochastic medi s razlichnymi fiziko-khimicheskimi kharakteristikami v organizm zhivotnykh // Vestnik OGU. № 2 (96). Orenburg. IPK GOU OGU 2009. S. 124 - 127.
13. Arsenteva I.P., Zotova E.S., Arsentev A.A., Glushhenko N.N., Bajjtukalov T.A., Folmanis G.EH. Ispolzovanie biologicheski aktivnykh nanoporoshkov na osnove magnija i zheleza v selskom khozjajjstve i medicine // Materialy VIII Vseros. konf. «Fizikokhimija ultradispersnykh (nano) sistem. (10-14 nojabrja g. Belgorod). M. MIFI. S. 258-260.
14. Bajjtukalov T.A., Glushhenko N.N., Bogoslovskaja O.A., Olkhovskaja I.P. Izuchenie vozdejjstvija nanochastic zheleza na soderzhanie gidroperoksidov v lipidakh pecheni v processe regeneracii kozhi posle nanesenija ehksperimentalnykh polnoslojjnykh ran // Sb. nauch. trudov II Vseros. nauch. konf. «Fiziko-khimicheskie i prikladnye problemy magnitnykh dispersnykh nanosistem». Stavropol. 2009. S. 276.
15. Kaletina N.I. Kaletin G.I. Mikroehlementy - biologi­cheskie reguljatory // Nauka v Rossii. izd. RAN. 2007. № 1. S. 50.
16. Skalnyjj A.V., Rudakov I.A. Bioehlementy v medicine. M.: Izdatelskijj dom «ONIKS 21 vek». Mir. 2004. 272 s.
17. Turconi G., Minoia C., Ronchi A., Roggi C. Dietary exposure estimates of twenty-one trace elements from a Total Diet Study carried out in Pavia Northern Italy // Br. J. Nutr. 2009. V. 101(8). P. 1200-1208.