M.V. Gavrilin, Yu.V. Mudretsova, S.V. Senchenko, S.A. Rozhnova

One of the most actual medical and social problems of the present are vascular diseases of a brain death rate from which in economically developed countries reaches 1112 %. Besides, the stroke also is one of the main reasons of the population invalidism. Nowadays for prevention and treatment of cerebrovascular diseases nootropic drugs are widely used. One of most widely used medicines with nootropic activity are choline alphoscerate and meldonium. Nowadays the works on development of the combined preparations containing choline alphoscerate and meldonium are carrying out. However, despite a wide application of these medicines methods of the analysis of these substances are developed not enough. In world practice for the analysis of similar substances a method of capillary electrophoresis (CE) is widely used. Using micellary electrokinetic chromatography with indirect absorbance detection the technique of a quantitative determination of choline alphoscerate and meldonium at combined presence was developed. As a leading electrolyte was used the binary buffer, containing solution of sodium tetra boras decahydrate (52 mM with addition of sodium dodecyl sulfate (75 mM) at рН 9,009,05. As choline alphoscerate and meldonium are substances that have no chromophore groups in their structure which can provide intensive absorbance of UV radiation, for their detection based on absorbance in UV spectrum area an indirect absorbance detection was applied. For this purposes the substance absorbing at 254 nm is entered into the leading electrolyte in the concentration providing necessary optical density of initial analyte. Optimal chromophore in this analysis conditions was acetylsalicylic acid with concentration 36 mM. It was shown that the developed technique has comprehensible values of linearity and precision.