M.V. Gavrilin, M.V. Larsky, S.P. Senchenko

This article is devoted to quantitative determination of the baicalin, the main active component of Scutellaria baicalensis. In this work we used samples of two series of Scutellaria extract, produced by Sichuan Sheng Shi Huakang Medicine Raw Material Factory (China). The analysis was performed using separation techniques HPLC and capillary electrophoresis (CE). Using a previously developed and validated HPLC method it was obtained that the average content of baicalin in the analyzed samples is within 87,5291,98 % with a relative error of determination <3 %. Searching of conditions for electrophoretic determination of baicalin in the Scutellaria extract was perfomed. It was found that the effective separation and optimal electrophoretic characteristics of baicalin peak observed when using 10 mg/ml borate electrolyte and 20 kV voltage. It is noted that the minimum relative error of the peak area obtained by using the standard sample solution with a concentration of baicalin 0,02 % (w/v). Two series of extract were analyzed under this conditions; it was found that the average content of baicalin is in the range 77,45 - 78,98 % (relative error of determination <5,4 %). Perhaps, the differences between results of HPLC and CE quantitative determination (8-14 %) are due to higher efficiency of the CE analysis.