D.G. Deryabin, N.A. Romanenko, T.G. Sviridova, G.I. El-Registan

Influence of four phenolic lipids - alkyloxybenzenes (AOB) on antibodies functional stability at a long-time storage in the water solution is investigated. As a criteria of antibodies stability is used the indicator of specific interaction with antigene and calculated time of loss 20 % antigen-binding abilities (TI20). For tested class G immunoglobulin to Toxoplasma gondii it is established time- and temperature-depending decrease of registered activity with TI20 = 44,0±3,3 days at +37 °С and TI20 = 351,1±7,0 days at +4 °С. The С3-АОB, С6-АОB and С12-АОB introduction in antibodies solutions led to initial suppression of their antigen-binding abilities, partially restored in dynamics of storage. Time and level of restoration depended from incubation temperatures, AOB homologues chemical structure and concentration, but not accompanied by increase in values TI20. The С1-АОB (1,3-dioxy-5-metilbenzol) introduction in antibodies solutions did not influence on antigen-binding ability but increased terms of this functional activity at +37°С (TI20 = 86,6±7,4 days; P < 0.05) without changes at +4 °С. The possible mechanisms of С1-АОB action by its influence on protein solvation are considered. This results are discussed in a context of alkyloxybenzenes prospects as stabilizers for diagnostic and therapeutic antibodies solutions.