350 rub
Journal №2 for 2012 г.
Article in number:
Isolation, Characterization and Biological Activity of Polysaccharides from Stellaria Media L
Keywords: Stellaria polysaccharides hepatoprotective activity
Authors:
Ya.V. Gorina, V.V. Golovchenko, Yu.S. Ovodov, E. V. Saprykina, E. A. Krasnov
Abstract:
The purpose of the present work is isolation of polysaccharide fractions from the aerial parts of Stellaria media L., establishment their basic composition and study of hepatoprotective activity. Three fractions of polysaccharides (SM 1, SM 2, SM 3) are isolated as a result, sequential extraction with water, acidified water (pH 4.0) and 0.7 % solution of ammonium oxalate from the aerial parts of Stellaria media L. It is established that the composition of carbohydrate chains of water-soluble polysaccharides fractions of SM 1 and SM 2 includes residues methoxylated galacturonic acid, glucose, galactose, arabinose, rhamnose, xylose and mannose. The polysaccharide component fraction SM 3 is presented by pectin polysaccharide with the primary content galacturonan. Fraction SM 1 containing water-soluble polysaccharides activates the regenerative-reparative processes at rats with experimental toxic hepatitis, with a daily for 6 days oral introduction at a dose (100 mg/kg) once a day. This indicates the presence of hepatoprotective activity similar to medicine «Carsil».
Pages: 9-15
References
  1. Лагерь А.А. Травник от всех болезней. М.: Рипол Классик. 2000. 672 с.
  2. Khare C. P. Indian Medicinal Plants: An Illustrated Dictionary. India. 2008. P. 900.
  3. Williamson E. M. Potter's Herbal Cyclopaedia: The Most Modern and Practical Book for All Those Interested in the Scientific As Well As the Traditional Use of Herbs in Medicine. Random House UK; Rev Sub edition. 2004. P. 528.
  4. Kitanov I. Phenolic acids and flavonoides from Stellaria media (L.) Vill (Caryophyllaceae) // Die Pharmazie. 1992. V. 47. № 6. P. 470-471.
  5. Bottger S., Melzig F. M. Triterpenoid saponins of the Caryophyllaceae and Illecebraceae family // Phytochemistry Letters. 2010. V. 32. № 6. P. 834-845.
  6. Shan Y., Zhou J., Zhao H.G., Feng X., Dong Y., Xia B. Amino acids and mineral composition of Stellaria media // Химия природных соединений. 2010. № 4. С. 562-563.
  7. Растительные ресурсы России: Дикорастущие цветковые растения, их компонентный состав и биологическая активность/ под ред. А.Л. Буданцева. СПб.: М. 2009. 513 с.
  8. Vanhaecke M., Ende W. V., Laere A. V., Herdewijn P., Lescrinier E. Complete NMR characterization of lychnose from Stellaria media (L.) Vill. // Carbohydrate Research. 2006. V. 341. № 16. P. 2744-2750.
  9. Vanhaecke M., Ende W. V., Lescrinier E., Dyubankova N. Isolation and Characterization of a Pentasaccharide from Stellaria media // Journal of National Products. 2008. № 71. P. 1833-1836.
  10. Vanhaecke M., Dyubankova N., Lescrinier E., Ende W. V. Metabolism of galactosyl-oligosaccharides in Stellaria media - Discovery of stellariose synthase, a novel type of galactosyltransferase // Phytochemistry. 2010. V. 71. P. 1095-1103.
  11. Leung M.Y.K., Liu C., Koon J. C. M., Fung K. P. Polysaccharide biological response modifiers // Immunology Letters. 2006. V. 105. P. 101-114.
  12. Togola A., Naess K. H., Diallo D., Barsett H., Michaelsen T. E., Paulsen B. S. A polysaccharide with 40% mono-O-methylated monosaccharides from the bark of Cola cordifolia (Sterculiaceae), a medicinal tree from Mali (West Africa) // Carbohydrate Polymers. 2008. V. 73. № 5. P. 280-288.
  13. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith, F. Colorimetric Method for Determination of Sugars and Related Substances // Analytical Chemistry. 1956. V. 28. Р. 350-356.
  14. Blumenkrantz N., Asboe-Hansen G. New method for quantitative determination of uronic acids // Analytical Biochemistry. 1973. V. 54. P. 484-489.
  15. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. Protein measurement with the Folin phenol reagent // The Journal of Biological Chemistry. 1951. V. 193. P. 265-275.
  16. Wood P. J., Siddiqui I. R. Determination of methanol and its application to measurement of pectin ester content and pectin methyl esterase activity // Analytical Biochemistry. 1971. V. 39. P. 418-428.
  17. York W.S., Darvil A.G., McNeil M., Stevenson T.T. Isolation and characterization of plant cell walls and cell-wall components // Methods in Enzymology. 1986. V. 118. P. 3-40.
  18. Hodge J. E., Hofreiter B. T. Determination of reducing sugars and carbohydrates // Methods in carbohydrate chemistry. New York. 1962. P. 380 - 394.
  19. Кочетков Н.К., Бочков А.Ф., Дмитриев Б.А., Усов А.И. и др. Химия углеводов. М. 1967. 672 c.
  20. Методы химии углеводов / под ред. Р.Л. Уистлера, Дж.Н. Бемиллер. М.: Мир. 1975. 445 с.
  21. Руководство по экспериментальным (доклиническим) исследованиям новых фармакологических веществ / под ред. Р.У. Хабриева. М. 2005. 836 с.
  22. Реброва О.Ю. Статистический анализ медицинских данных. Применение прикладных программ STATISTICA. М. 2002. 305 c.