V.L. Beloborodov, N.G. Zakharova, A.M. Savvateev, V.K. Kolkhir, I.V. Voskoboynikova

Phyto Novo-Sed is a multi-component Russian herbal drug containing extract of a mixture of Motherwort (Leonurus cardiaca L.); lemon balm (Melissa officinalis L.); hawthorn (Fructus Crataegi), cinnamon rose (Fructus Rosae), purple coneflower (Herba Echinacea purpureae (L.) Moench) in mass parities 2:2:1:2:1. A HPLC method for qualitative analysis of drug components and the all kinds of herbal extracts has been developed. The optimum chromatographic conditions for HPLC determination of components of the herbal drug were realized. The separation of extract components was satisfactorily resolved by using a octadecylsilane adsorbent and gradient elution with mixture of acetonitrile, tetrahydrofurane, phosphate buffer and dimethylformamide. HPLC analysis was optimized according to chromatographic parameters. The 17 biologically active components of the Phyto Novo-Sed was identify. Нerbal drug containing wide range of polyphenols, such as flavonoids (aglycons and glycosides) and hydroxycinnamates. Indicator substances for each herbal component have been found, and a possibility of their use as a markers for herbal component identification has been proved. A TLC techniques to analysis indicator substances were realized. The chromatography methods can be used for drug standardization as the ways to confirm the identity of the herbal drug «Phyto Novo-Sed».