350 rub
Journal №9 for 2010 г.
Article in number:
Study of biological addiTIVe "Bioscan C" genetic effects in Ames test
Keywords: mutagenic activity the biological additive Bioscan C dihydroquercetin antimutagenic action
Authors:
V.N. Zinovyeva, A.A. Spasov
Abstract:
Antioxidant intake into diet is known to decrease cancer risk because antioxidants reduce level of free oxygen species and DNA damage induced them. Biological additives based on antioxidants with anticarcinogenic/antimutagenic activity may be used for cancer prophylactic. Plant flavonoid antioxidants have large spectrum of biological action and are included into many biological additives. However several flavonoids act as mutagens, so it-s important to study the mutagenicity of flavonoid biological additives. We investigated the capacity of the biological additive Bioscan C that contains antioxidant flavanone dihydroquercetin to induce gene mutations in Ames test on Salmonella typhimurium ТА100, ТА98 и ТА97 strains. Bioscan C at low concentrations (0,1 - 100 μg on plate) was ineffective in the presence or the absence of mammalian metabolic activation. Bioscan C at high concentrations (1000 - 5000 μg on plate) induced a number of reverse mutants considered statistically significant. With metabolic activation the mutagenic activity of Bioscan C was lower then without it. There are some association between antioxidant property of chemicals and their antimutagenic action. We studied the capacity of Bioscan C to reduce genotoxic activity of dioxidin, furagin, sodium azide and potassium dichromate, but it was not effective against these direct mutagens in Ames test.
Pages: 18-22
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