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Acceleration of heart function recovery by peptide IX (29–40 MCP-1) after myocardial ischemia-reperfusion in rats

DOI 10.18127/j20700997-201903-02

Keywords:

M.R. Akhmetshina –
Assistant, Department of Physiology and General Pathology, Faculty of Fundamental Medicine, Lomonosov Moscow State University
E-mail: akhmetshinamar@gmail.com
M.P. Morozova –
Ph.D. (Biol.), Assistant, Department of Physiology and General Pathology, Faculty of Fundamental Medicine, Lomonosov Moscow State University
S.A. Gavrilova –
Ph.D. (Biol.), Department of Physiology and General Pathology, Faculty of Fundamental Medicine, Lomonosov Moscow State University
E.V. Lukoshkova –
Dr.Sc. (Biol.), Research Scientist, Institute of Experimental Cardiology, Russian Cardiology Research Complex Ministry of Health of Russia
M.V. Sidorova –
Ph.D. (Chem.), Head of Laboratory, Institute of Experimental Cardiology, Russian Cardiology Research Complex
Ministry of Health of Russia
T.L. Krasnikova –
Dr.Sc. (Biol.), Institute of Experimental Cardiology, Russian Cardiology Research Complex, Ministry of Health of Russia
V.B. Koshelev –
Dr. Sc. (Biol.), Professor, Head of Department of Physiology and General Pathology, Faculty of Fundamental Medicine,
Lomonosov Moscow State University


In this paper the effect of peptide IX, a structural fragment of MCP-1 (which accelerates and intensifies inflammation during early periods following ischemia-reperfusion), on the cardiac performance 72 hours and 28 days after a myocardial infarction in rats was examined. Ligation of the left coronary artery was used to model a myocardial infarction. Peptide IX (saline for control group) was administered via intracardiac injection at the time of surgery. After 2.5 hours, reperfusion was performed. Parameters of hemodynamics were evaluated 72 hours and 28 days after the operation: heart rate, mean arterial pressure, systolic and diastolic pressure in the left ventricle of the heart, indices of myocardial contractility and lusitropy at rest and in response to the administration of dobutamine. We studied changes in regulation of heart function from autonomic nervous system at rest and in response to exposure to cold prior to surgery and in cases where the peptide was administrated 24 hours, 72 hours and 28 days after ischemia-reperfusion. Peptide IX showed cardioprotective effect during early periods after the operation. In response to the administration of dobutamine heart rate reactions and myocardial contractility intensified, the lusitropy was partially restored. In the long-time period after the operation the differences between the intact control group and the peptide group were imperceptible. Thus, the acceleration of inflammation during ischemia-reperfusion due to the effect on monocytes and macrophages demonstrates an early cardioprotective effect. Further research is required to develop a concept of controlled inflammation during myocardial infarction.

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