D.Yu. Izmailov – Ph.D. (Biol.), Associate Professor, Department of Medical Biophysics, Faculty of Funda-mental Medicine, Lomonosov Moscow State University
E.V. Proskurnina – Ph.D. (Chem.), Associate Professor, Department of Medical Biophysics, Faculty of Fun-damental Medicine, Lomonosov Moscow State University
E.M. Demin – Post-graduate Student, Department of Medical Biophysics, Faculty of Fundamental Medicine, Lomonosov Moscow State University
Yu.A. Vladimirov – Dr. Sc. (Biol.), Professor, Full Member of the Russian Academy of Sciences, Head of the Department of Medical Biophysics, Faculty of Fundamental Medicine, Lomonosov Moscow State University, Lomonosovsky
The effect of several antioxidants (quercetin, taxifolin, rutin, trolox, ascorbate, ionol, α-tocopherol, desferal, probucol, na-ringenin, mexidol) on the oxidation of luminol by hydrogen peroxide catalyzed by the cytochrome c complex with 1,1',2,2' -tetraoleoylcardiolipin (TOCL). When cytochrome c is added to a solution containing TOCL, luminol and H2O2, a chemilumi-nescence response develops, the amplitude and light sum of which decrease dose-dependently in the presence of antioxi-dants.
Computer simulation of kinetics of peroxidase reaction catalyzed by a complex of cytochrome c with TOCL in the presence of antioxidants was carried out. It has been established that the effect of antioxidants on the chemiluminescence kinetics can be described by a single general reaction of the interaction of antioxidants with free radicals. The term "antioxidant activity" is specified. It is a constant of the rate of interaction of antioxidants with free radicals. For the antioxidants studied, the rate constants are calculated and a classification is proposed for weak, strong and moderate, depending on this parameter. Strong antioxidants in the studied system were trolox and ascorbate (k >> 1), moderate antioxidants: alpha-tocopherol, rutin, quercetin, taxifolin (k ~ 1-2), weak antioxidants: ionol, mexidol, probucol, naringenin, desferal (k<1).
- Belikova N.A., Vladimirov Y.A., Osipov A.N., Kapralov A.A., Tyurin V.A., Potapovich M.V., Basova L.V., Peterson J., Kurnikov I.V., Kagan V.E. Peroxidase activity and structural transitions of cytochrome c bound to cardiolipin-containing membranes // Biochemistry. 2006. V. 45. № 15. P. 4998–5009.
- Iwase H., Takatori T., Nagao M., Nijima H., Iwadate K., Matsuda Y., Kobayashi M. Formation of keto and hydroxy compounds of linoleic acid in submitochondrial particles of bovine heart // Free Radic Biol Med. 1998. V. 24. № 9. P. 1492–1503.
- Stepanov G., Gnedenko O., Mol'nar A., Ivanov A., Vladimirov Y., Osipov A. Evaluation of cytochrome c affinity to anionic phospholipids by means of surface plasmon resonance // FEBS Lett. 2009. V. 583. № 1. P. 97–100.
- Kagan V.E., Borisenko G.G., Tyurina Y.Y., Tyurin V.A., Jiang J., Potapovich A.I., Kini V., Amoscato A.A., Fujii Y. Oxidative lipidomics of apoptosis: Redox catalytic interactions of cytochrome c with cardiolipin and phosphatidylserine // Free Radic Biol Med. 2004. V. 37. № 12. P. 1963–1985.
- Izmailov D.Y., Vladimirov Y.A. The mathematical modeling of the kinetics of chain lipid peroxidation in the presence of fe2+: Main model. // Biologicheskije Membrany (Rus). 2002. V. 19. № 6. P. 507–515.
- Izmailov D.Y., Vladimirov Yu A. The mathematical modeling of the kinetics of chain lipid peroxidation in the presence of fe2+. Ii. Effect of antioxidants // Biologicheskije Membrany (Rus). 2003. V. 20. № 4. P. 349–358.
- De Pinto M.C., Ros Barcelo A. Inhibition of both peroxidase and laccase by desferal (desferrioxamine mesylate) // Phytochemistry. 1996. V. 42. № 2. P. 283–286.