Radiotekhnika
Publishing house Radiotekhnika

"Publishing house Radiotekhnika":
scientific and technical literature.
Books and journals of publishing houses: IPRZHR, RS-PRESS, SCIENCE-PRESS


Тел.: +7 (495) 625-9241

 

Effects of antioxidants on peroxidase reaction catalyzed by complex of cytochrome c with cardiolipin: application of computer simulation

Keywords:

D.Yu. Izmailov – Ph.D. (Biol.), Associate Professor, Department of Medical Biophysics, Faculty of Funda-mental Medicine, Lomonosov Moscow State University
E-mail: dizm@mail.ru
E.V. Proskurnina – Ph.D. (Chem.), Associate Professor, Department of Medical Biophysics, Faculty of Fun-damental Medicine, Lomonosov Moscow State University
E-mail: proskurnina@gmail.com
E.M. Demin – Post-graduate Student, Department of Medical Biophysics, Faculty of Fundamental Medicine, Lomonosov Moscow State University
E-mail: adamantane@yandex.ru
Yu.A. Vladimirov – Dr. Sc. (Biol.), Professor, Full Member of the Russian Academy of Sciences, Head of the Department of Medical Biophysics, Faculty of Fundamental Medicine, Lomonosov Moscow State University, Lomonosovsky
E-mail: yuvlad@mail.ru


The effect of several antioxidants (quercetin, taxifolin, rutin, trolox, ascorbate, ionol, α-tocopherol, desferal, probucol, na-ringenin, mexidol) on the oxidation of luminol by hydrogen peroxide catalyzed by the cytochrome c complex with 1,1',2,2' -tetraoleoylcardiolipin (TOCL). When cytochrome c is added to a solution containing TOCL, luminol and H2O2, a chemilumi-nescence response develops, the amplitude and light sum of which decrease dose-dependently in the presence of antioxi-dants.
Computer simulation of kinetics of peroxidase reaction catalyzed by a complex of cytochrome c with TOCL in the presence of antioxidants was carried out. It has been established that the effect of antioxidants on the chemiluminescence kinetics can be described by a single general reaction of the interaction of antioxidants with free radicals. The term "antioxidant activity" is specified. It is a constant of the rate of interaction of antioxidants with free radicals. For the antioxidants studied, the rate constants are calculated and a classification is proposed for weak, strong and moderate, depending on this parameter. Strong antioxidants in the studied system were trolox and ascorbate (k >> 1), moderate antioxidants: alpha-tocopherol, rutin, quercetin, taxifolin (k ~ 1-2), weak antioxidants: ionol, mexidol, probucol, naringenin, desferal (k<1).

References:
  1. Belikova N.A., Vladimirov Y.A., Osipov A.N., Kapralov A.A., Tyurin V.A., Potapovich M.V., Basova L.V., Peterson J., Kurnikov I.V., Kagan V.E. Peroxidase activity and structural transitions of cytochrome c bound to cardiolipin-containing membranes // Biochemistry. 2006. V. 45. № 15. P. 4998–5009.
  2. Iwase H., Takatori T., Nagao M., Nijima H., Iwadate K., Matsuda Y., Kobayashi M. Formation of keto and hydroxy compounds of linoleic acid in submitochondrial particles of bovine heart // Free Radic Biol Med. 1998. V. 24. № 9. P. 1492–1503.
  3. Stepanov G., Gnedenko O., Mol'nar A., Ivanov A., Vladimirov Y., Osipov A. Evaluation of cytochrome c affinity to anionic phospholipids by means of surface plasmon resonance // FEBS Lett. 2009. V. 583. № 1. P. 97–100.
  4. Kagan V.E., Borisenko G.G., Tyurina Y.Y., Tyurin V.A., Jiang J., Potapovich A.I., Kini V., Amoscato A.A., Fujii Y. Oxidative lipidomics of apoptosis: Redox catalytic interactions of cytochrome c with cardiolipin and phosphatidylserine // Free Radic Biol Med. 2004. V. 37. № 12. P. 1963–1985.
  5. Izmailov D.Y., Vladimirov Y.A. The mathematical modeling of the kinetics of chain lipid peroxidation in the presence of fe2+: Main model. // Biologicheskije Membrany (Rus). 2002. V. 19. № 6. P. 507–515.
  6. Izmailov D.Y., Vladimirov Yu A. The mathematical modeling of the kinetics of chain lipid peroxidation in the presence of fe2+. Ii. Effect of antioxidants // Biologicheskije Membrany (Rus). 2003. V. 20. № 4. P. 349–358.
  7. De Pinto M.C., Ros Barcelo A. Inhibition of both peroxidase and laccase by desferal (desferrioxamine mesylate) // Phytochemistry. 1996. V. 42. № 2. P. 283–286.
May 29, 2020

© Издательство «РАДИОТЕХНИКА», 2004-2017            Тел.: (495) 625-9241                   Designed by [SWAP]Studio