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Free-radical producingfunction of neutrophils of patients with myelodysplastic syndrome: diagnostics using kinetic chemiluminescence with two-step stimulation and clinical relevance

Keywords:

Elena Vasil’evna Proskurnina – Ph.D. (Chem.), Associate Professor, Department of Medical Biophysics, Faculty of Funda-mental Medicine, Lomonosov Moscow State University
E-mail: proskurnina@gmail.com
Galina Anatol’evna Dudina – Ph.D. (Med.), Head of the Department of Oncohematology, Loginov Moscow Clinical Scientific Center
E-mail: dudina_gal@mail.ru
Madina Magamedovna Sozarukova – Ph.D. (Biol.), Department of Biochemistry of Free-radical Processes, Myasnikov Institute of Clinical Cardiology
E-mail: а.s_madinam@bk.ru
Lyudmila Robertovna Orlova – Physician of the City Clinic No. 191 of the Moscow Healthcare Department, Moscow
E-mail: dius.eilin@rambler.ru
Yulia Alexandrovna Filippova – Student, Faculty of Fundamental Medicine, Lomonosov Moscow State University
E-mail: yulia.philippova@mail.ru
Maya Andreevna Shestakova – Student, Pirogov Russian National Research Medical University
(Moscow)
E-mail: chrysolite7@gmail.com
Aleksandr Vasil’evich Pivnik – Dr. Sc. (Med.), Professor, Head of the Scientific Department of Oncohematology and Secondary Immunodeficiency Diseases, Loginov Moscow Clinical Scientific Center


To evaluate the radical-producing function of neutrophils, the method of luminol-enhanced chemiluminescence with double-step activation (4β-phorbol-12-myristate-13 acetate followed by N-formyl-methionyl-leucyl-phenylalanine) was used. The activation coefficients for luminol-dependent chemiluminescencewere calculated as analytical parameters for the following groups of patients: 1) almost healthy donors, 2) patients with myelodysplastic syndrome (MDS), low risk, 3) MDS, intermediate 1 risk, 4) MDS, intermediate 2 risk, 5) MDS, high risk, in including patients with transformation into acute myeloid leukemia. These parameters were also calculated for patients before and after blood transfusion. It is shown that as the disease progresses, the activation coefficient decreases. Thus, this parameter of the radical-producing function of neutrophils can serve as a diagnostic and prognostic indicator for assessing the severity of the disease and the selection of the protocol of blood transfusion therapy.

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May 29, 2020

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