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Major features of ultrasonic impact on plateletes

Keywords:

A.A. Oleshkevich - Dr.Sc. (Biol.), Associate Professor, Department of Informational Technologies, Mathematics and Physics, Moscow State Academy of Veterinary Medicine and Biotechnology  MVA by K.I. Skryabin
E-mail: kompsotita@gmail.com
T.V. Ippolitova - Dr.Sc. (Biol.), Professor, Head of the Department of Physiology and Pharmacology, Moscow State Academy of Veterinary Medicine and Biotechnology  MVA by K. I. Skryabin (Moscow SAVMB)
E-mail: ippolitova01@mail.ru


Physiological, biophysical and morphological studies on platelets of domestic animals have been carried out. The ultrasonic carrier frequency was 0.88 MHz or 2.64 MHz, therapeutic levels of intensity ISATA  average over space and time intensity  were from 0.05 W/cm2 to 1.0 W/cm2. The active modulation frequency ranged in 10–150 Hz and 700–1000 Hz. The irra-diated blood volume from dogs and cats ranged from 1 to 1.5 ml. Destructive, cytolytic and many other effects of continuous and pulse-modulated ultrasound were detected. The spectra of active frequencies and intensities affecting the platelets’ state in vitro are revealed. The possibility of changing of staining methods for the entire population of blood cells after directing the ultrasound of the blood platelets was tested. Positive results of changes in the structure of the cytoplasmic membrane resulting in a deep simultaneous staining of blood cells (RBC, WBC, platelets) were obtained. Platelets were stained after blood sonication with traveling continuous mode US wave from 20 to 45 sec with the intensity of 0.4 W/cm2, followed by the preparation of blood smears and differential coloring dyes.
The dyeing of platelets was performed after US exposure on blood samples. Depending on the amplitude-modulated US in-tensity different exposure was used. With 0.05 W/cm2 intensity we treated for 30–40 sec, with intensity of 0.2 W/cm2  20–35 sec, of 0.4 W/cm2  15–30 sec, and with 0.7 W/cm2  for 10–20 sec (The carrier frequency was 0.88 MHz). At the same time, we imposed on these modes any number of the 10 – 30 Hz modulation frequency range or 800 Hz, the duty cycle 2. The same result can be achieved using the carrier frequency of 2.64 MHz & the intensity of 0.4 W/cm2 (exposure time 15–30 sec).
Our findings can be used for non-invasive animals’ blood test.

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May 29, 2020

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