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Cryopreservation of cyanobacteria arthrospira platensis

Keywords:

D.I. Petrukhina - Laboratory Assistant, Department of Botany, Microbiology and Ecology, Institute of Natural Sciences, Tsiolkovsky Kaluga State University
E-mail: daria.petrukhina@outlook.com
I.N. Lykov - Ph.D. (Med.), Dr.Sc. (Biol.), Professor, Head of Department of Botany, Microbiology and Ecology, Scientific Adviser of Institute of Natural Sciences, Tsiolkovsky Kaluga State University
E-mail: linprof47@yandex.ru


The aim of the current research was testing the possibilities of low-temperature storage of some strains Arthrospira platensis obtained from different collections of cultures. The major criterion of low-temperature storage was its applicability for all strains Arthrospira platensis in question. To this end, glucose as a cryoprotectant was used along with determination of its optimal concentration. Influence of partner organisms of two un-axenic strains Arthrospira platensis was investigated with. This partner organisms - bacteria and micro fungi - are present in unialgal cultures due to co-isolation while creating culture collections of microalgae and cyanobacteria [10]. Furthermore, it was observed macroscopic morphology of growth on agar plate all tested strains Arthrospira platensis. In the present research, all tested strains Arthrospira platensis were viable after storage for one month under constant temperature of -80°С in a glucose solution as a cryoprotectant at concentrations of 5 and 15%. It was observed that 15% glucose solution provoked proliferation of the partner organism in cultures of un-axenic strains Arthrospira platensis which led to bacterial contaminating in the recultivated cultures. On the other hand, with 5 to 10% glucose solution the contamination in un-axenic cyanobacteria’s cultures was not observed whereas the highest growth rates of strains Arthrospira platensis after thawing were achieved with 10% glucose.

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May 29, 2020

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