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The model describing the fluctuation of extracellular DNA properties after exposure of cells to the adversive factors

Keywords:

R.V. Veiko – Junior Research Scientist, Research Centre for Medical Genetics (Moscow)
E-mail: db-mail-dbase@yandex.ru
E.M. Gestkova – Doctor-psychiatrist, Psychiatric Hospital №14, Department of Healthcare of Moscow
E-mail: info@pb14.mosgorzdrav.ru
E.S. Ershova – Leading Research Scientist, Research Centre for Medical Genetics (Moscow)
E-mail: ershova@rambler.ru
N.N. Veiko – Dr.Sc. (Biol.), Chief Research Scientist, Research Centre for Medical Genetics (Moscow)
E-mail: satelit32006@yandex.ru
P.E. Umriukhin – Dr.Sc. (Med.), Professor, I.M. Sechenov First Moscow State Medical University; Senior Re-search Scientist, P.K. Anokhin Institute of Normal Physiology (Moscow)
E-mail: pavelum@mail.ru
S.V. Kostyuk – Dr.Sc. (Biol.), Head of Molecular Biology Laboratory, Research Centre for Medical Genetics (Moscow)
E-mail: svet-vk@yandex.ru


The article describes an application of information technologies for the modeling of biological processes in the cells of living or-ganisms: the effects of radiation or of the oxidative stress in particular. There are three groups of cells may be found after the exposure: the first group irrevocably dies, the second group stays healthy and the third group consists of cells which able to re-paration. Belonging to one of these groups depends on the intensity of the damaging effect and the threshold of number of breaks in the DNA. The cell dies (apoptosis) if the number of breaks is more than this value. During the apoptosis the dying cells secrete DNA into the extracellular space. This DNA is called the extracellular DNA. Thus, the amount of extracellular DNA directly depends on the number of dead cells. Over the last 10 years the laboratory of molecular biology of Research Center of Medical Genetic explores the extracellular DNA. We have found some extracellular DNA correlations with the peculiarities of different pa-thologic processes. For example, the amount of extracellular DNA in schizophrenic patients is higher than in healthy control. In this article we describe the computer model developed for our research. This model simulates cellular death and its conse-quences after cells exposure to the adversive factors. The parameters are set statically before simulation. The second group of parameters may change during the simulation. The model works under Windows operating system (Windows XP and later). This application consist of implementation of data structures and algorithms and graphical user interface (GUI) for entering the para-meters and view the results of simulation. The GUI should support Russian and English languages. The results of simulation are represented in a form of a table. For further processing the data may be transferred to Microsoft Excel sheet. The results of computer simulation fairy match to the experimental results received in our laboratory. The program was written in Object Pascal (Turbo Delphi 2006 IDE). The computer science used to develop and implement:
compilation theory (the calculation of functional dependencies);
computer simulation of biological systems (simulation of cells properties and apoptosis).
This article contains the description of algorithms. For more details the author contact information may be used. The comparison of received results by the simulation with experimental data is described in the discussion of a current article.

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