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Study of the effect of certain frequencies of visible light and EHF radiation on the viability of eggs of loach (Misgurnis fossilis) in the process of embryogenesis

Keywords:

A.H. Tambiev - Dr.Sc.(Biol.), Professor, M.V. Lomonosov Moscow State University A.N. Velikanov - Leading Engineer, M.V., Lomonosov Moscow State University. E-mail: av-bioem@mail.ru N.N. Vorobyeva - Research Scientist, Institute on Laser and Information Technologies of the Russian Academy of Sciencies (ILIT RAS) (Moscow, Troitsk) O.V. Burlakova - Ph.Dr (Biol.), Leading Research Scientist, M.V. Lomonosov Moscow State University E.G. Korvin-Pavlovskaya - Ph.Dr (Biol.), Senior Research Scientist, M.V. Lomonosov Moscow State University. V.A. Golichenkov - Dr.Sc.(Biol.), Professor, M.V. Lomonosov Moscow State University


In the present work investigated the effect of continuous visible light (LEDs λ = 470, 630, diode laser of 660 nm) and pulsed short-wave radiation (λ = 7.1 mm) in the progression of stages of embryogenesis in loach embryos (Misgurnis fossilis). Within 20 minutes after fertilization, eggs were seeded in 35 mm Petri dishes of 50 embryos in each, and then when thermostating temperature of 18oC. We used about 1200 loach embryos in each experiment. The radiation produced during the crushing of eggs and at the stage of blastula within 60 minutes, with the power density for all used in this work was equal and amounted to 2 mW/cm2. It was interesting to compare the influence of EHF-radiation on development of eggs of loach with the action of visible light for a range of wavelengths. Upon irradiation of λ = 660 and 630 nm of embryos at the blastula stage was observed stimulation of viability, expressed as the increase in the number of living embryos that have passed gastrulation, respectively, 16% and 24%. The action of the wavelengths of the red end of the visible light (λ = 630 and 660 nm) was dependent on the stage of development of loach embryos. EHF-radiation and visible light with a wavelength of 470 nm showed no pronounced effect that it is possible to explain selective action of the used wavelengths of visible light and short-wave radiation at a certain stage of embryogenesis (uninstalling).
References:

 

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