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Toxic effect of Polyoxometalates (POMs) with Keggin’s structure on normal and tumor cells culture

Keywords:

O.A. Lopatina – Ph.D.(Med.), Leading Research Scientist, Federal Research N.F Gamaleya Center of Epidemiology and Microbiology D.I. Ivanovsky Institute of Virology O.V. Baklanova – Ph.D.(Biol.), Senior Research Scientist, Federal Research N.F Gamaleya Center of Epidemiology and Microbiology D.I. Ivanovsky Institute of Virology I.A. Suetina – Ph.D.(Biol.), Leading Research Scientist, Federal Research N.F Gamaleya Center of Epidemiology and Microbiology D.I. Ivanovsky Institute of Virology G.R. Mikhailova – Ph.D.(Biol.), Leading Research Scientist, Federal Research N.F Gamaleya Center of Epidemiology and Microbiology D.I. Ivanovsky Institute of Virology E.A. Guchina – Ph.D.(Biol.), Leading Research Scientist, Federal Research N.F Gamaleya Center of Epidemiology and Microbiology D.I. Ivanovsky Institute of Virology F.A. Lisicin – Ph.D.(Biol.), Leading Research Scientist, Federal Research N.F Gamaleya Center of Epidemiology and Microbiology D.I. Ivanovsky Institute of Virology L.I. Russu – Research Scientist, Federal Research N.F Gamaleya Center of Epidemiology and Microbiology D.I. Ivanovsky Institute of Virology S.A. Kovalevskyi Junior Research Scientist, N.N. Semenov Institute of Chemical Physics, RAS B.A. Budanov – Dr.Sc.(Phys.-Math.), Professor, N.N. Semenov Institute of Chemical Physics, RAS F.I. Dalidchik – Dr.Sc.(Phys.-Math.), N.N. Semenov Institute of Chemical Physics, RAS M.V. Mezentzeva – Dr.Sc.(Biol.), Federal Research N.F Gamaleya Center of Epidemiology and Microbiology D.I. Ivanovsky Institute of Virology


Polyoxometalates (POMs) are stable metal-oxide inorganic compounds. Their structure and electronic properties can be modified. They are one of the perspective products of nanotechnology in medicine and bioengineering, in-cluding antiviral and antitumor drug development. In this context, it is required to investigate POM’s cytotoxity on cells culture. Objective: to investigate biological processes of different POM samples on cells culture: human embryo fibroblast (HEF), human lung embrio (HLE) and human lung carcinoma A-549. POM cytotocxic effect on HEF cell was investigated by MTT-test. Growth activity was measured by proliferative index and cells vitality. Cells morphology was investigated by atomic-force (AFM) and electron microscopes (EM). Results: POM samples demonstrated various toxic effects on cells cultures. POMs (H3PW12O40, H4SiMo12O40. H3PMo12O4 ) reduced cells growth activity and led to significant morphology changes, seen in AFM and EM. Cells destruction and deterioration with failure of cytoplasm membrane structures and karyolysis, up to cells necrosis. Comparison between proliferation index and reduction potential after the 4th passage of HLE cells was realized. Correlation between proliferation index and reduction potential also was found. The most toxic samples were POM A – H3PW12O40 and POM C – H4SiW12O40. They had the lowest reduction potential. It was identified that IC50 se-quence corresponded reduction potentials. It provides the possible ways for potential medicine creation.
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