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Membranotropic effects of ethanol: changes of potentials and ionic currents of neurons of snails

Keywords:

V. I. Orlov – Senior Research Scientist, A.B. Kogan RSI of neurocybernetics, Southern Federal University (Rostov-on-Don). E-mail: orlov@rostel.ru
A. I. Vislobokov – Dr.Sc. (Biol.), Head of the Department of Neural Pharmacology, Senior Research Scientist, A.V. Waldman Institute of Pharmacology, Academician I.P. Pavlov PSPbSMU (St. Petersburg). E-mail: vislobokov@yandex.ru
E. A. Marutkina – Under-graduate Student of Specialized Master's Program "Bio and Neuroinformatics" of Biological Academy and Biotechnology, A.B. Kogan RSI of neurocybernetics, Southern Federal University (Rostov-on-Don). E-mail: r-dance@mail.ru
A. G. Sukhov – Dr.Sc. (Biol.), Head of the Department, A.B. Kogan RSI of neurocybernetics, Southern Federal University (Rostov-on-Don). E-mail: w701@krinc.ru


The paper describes the microelectrode studies and voltage clamp technique which show that the ethanol in concentrations of 1, 10, 100, 250, 500, 1000, and 2000 mM by extracellular application changes electrical activity of identifiable neurons of pedal ganglia snails of Planorbarius corneus and sodium, calcic and potassium ionic currents. In concentrations of 1, 10, and 100 mM ethanol didn’t change resting potential or hyperpolarized neurons, without changing ionic currents. In concentrations of 250, 500, 1000, and 2000 mM (1,12…9% solutions) ethanol depolarized neurons, and against this background the frequency and duration of action potential were increasing and their amplitude and total ionic currents were decreasing. Authors observe the acceleration of inactivation of calcic currents. Changes were completely reversible, when laundering the hyperpolarization of cells arose, in authors’ opinion, due probably to activation of electrogenic transport of sodium ions. Authors suggest that under the influence of ethanol the changes of state of neurons caused not only by changes of resting potential, but also the direct effect on ion channels. The conclusions of relative safety of ethanol in concentrations of 100 mM using for dissolution of pharmacological agents for biological objects were made.
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