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Dose-dependent effect of Tactivin on the behavior of rats and nucleoli number in the neurons of some brain structures

Keywords:

A. V. Kryuchkova – Junior Researcher, FSBIS SRI PCM FMBA of Russia (Moscow)
A. N. Inozemtsev – Dr.Sc. (Biol.), Leading Research Scientist, Chief of Laboratory, Lomonosov Moscow State University (Moscow)
V. Ya. Arion – Dr.Sc. (Biol.), Professor, Principal Research Scientist, FSBIS SRI PCM FMBA of Russia (Moscow)
E. V. Loseva – Dr.Sc. (Biol.), Principal Research Scientist, FSBI IHNA&NPh RAS (Moscow)


In this paper, we investigated the effects of two doses of Tactivin (peptide immunoactive drug of bovine thymus) intranasal administration on animal behavior and the number of nucleoli in the neurons of three structures of rat brain. Animals were intranasally administered with small and large dose of tactivin (0.05 mcg and 1 mcg per rat pro tanto). Three tests were used: “open field” (OF), “dark light camera” (DLC) and “elevated plus maze” (EPM). The analysis of nucleoli number was carried out in pyramidal layer of CA1 hippocampal field, in pyramidal neurons of V layer of somatosensory cortex, and in the head of the caudate nucleus. It was found that animals given a small dose of Tactivin slightly increased locomotor and exploratory activity in the DLC and EPM tests, which could indicate a reduction of anxiety level. Animals receiving the high dose of the drug, showed a pronounced decrease of habituation in the OF-test, anxiety and depressive behavior in EPM. It was found that long (18 days) injection of both Tactivin doses decrease the nucleoli number in pyramidal layer of CA1 hippocampal field, in pyramidal neurons of V layer of somatosensory cortex, but not in the head of the caudate nucleus. The obtained data allow to think that Tactivin shows a pronounced dose-dependent effect on behavior of animals in used tests. However, it is shown specific but not dose-dependent impact of Tactivin on nucleoli number in neurons of investigated structures of rat brain. The reported study was partly supported by RFBR, research project No.14-04-32087 mol_a.
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