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Methodical approach specific indication of avian influenza A H5N1 by dielectrophoresis

Keywords:

V.M. Generalov – Ph.D. (Eng.), Leading Research Scientist, State Research Center of Virology and Biotechnology VECTOR (Koltsovo, Novosibirsk region). E mail: general@vector.nsc.ru
A.G. Durymanov – Senior Research Scientist, State Research Center of Virology and Biotechnology VECTOR. E mail: shestopalov@vector.nsc.ru
K.V. Generalov – Engineer-programmer, State Research Center of Virology and Biotechnology VECTOR. E mail: gvk@vector.nsc.ru
M.V. Kruchinina – Dr. Sc. (Med.), Leading Research Scientist, Scientific Research Institute of Therapy, Siberian Branch of RAMS (Novosibirsk) E mail: kruchmargo@yandex.ru
O.G. Kurskaya – Research Scientist, State Research Center of Virology and Biotechnology VECTOR. E mail: kurskaya_og@vector.nsc.ru
G.A. Buryak – Research Scientist, State Research Center of Virology and Biotechnology VECTOR (Koltsovo, Novosibirsk region). E mail: buryak@vector.nsc.ru
A.S. Safatov – Dr. Sc. (Eng.), Head of Department, State Research Center of Virology and Biotechnology VECTOR. E mail: safatov@vector.nsc.ru


The paper presents materials on the methodological approach involving the use of dielectrophoresis for specific indication of influenza virus A/Common gull/Chany/P/2006 (H5N1). Indication was performed in the frequency range (50 – 2000) kHz of non uniform alternating electric field (NAEF) and the voltage U = 5 V. Equine erythrocytes, specific serum sensitive to the above-mentioned virus and normal (non-sensitive) serum were used in the study. Indication was based on the effect of neutralizing the toxic action of the virus by specific serum. As a result, erythrocytes in the NAEF behave like intact ones. Indication is recorded by the collective behavior of erythrocytes in NAEF, the presence of negative and positive dielectrophoresis, as specified for the four test-tubes described below. Test-tube 1. The presence of negative and positive dielectrophoresis of intact - control equine erythrocytes is verified. Test-tube 2. The presence of negative dielectrophoresis at a frequency of 50 kHz and positive dielectrophoresis at a frequency of 2000 kHz for equine erythrocytes is verified after adding influenza virus. Test-tube 3. The presence of negative dielectrophoresis at a frequency of 50 kHz and positive dielectrophoresis at 2000 kHz for equine erythrocytes is verified after adding a suspension of influenza virus with specific antiserum. Test-tube 4. The presence of negative dielectrophoresis at a frequency of 50 kHz and negative dielectrophoresis at 2000 kHz for equine erythrocytes is verified after adding a suspension of influenza virus with normal serum. Neutralization of the toxic effect of the virus on erythrocytes by specific serum does not take place. The total indication time taking into account the time required for sample preparation is about 30 minutes.
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