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Mycoplasma increased resistance of H292 lung cancer cell line to Nutlin3 through activation of nfkb signaling pathway


U.A. Boyarskih, D.A. Zhechev, V.V. Kozlov, M.A. Smetanina, A.E. Kel, M.L. Filipenko

Nutlin3 is an antitumor compound that selectively induces cell cycle arrest and death of tumor cells through p53 reactivation. We examined effect of Mycoplasma hyorhinis on the sensitivity of H292 cells to Nutlin3. Mycoplasma infection reduces the cytotoxic effects of Nutlin3 on NCI-N292 cells (in cell culture infected with M. hyorhinis the percentage of viable cells after incubation with 30 µM Nutlin3 was significantly higher than in uninfected cell culture; 11,5±5,2 vs 59,5±11,4; p<0,001). Additionally, еpithelial mesenchymal transformation of cell culture was a concomitant effect of mycoplasma infection. Microarray-based transcriptome analysis of infected and uninfected cells revealed the activation of IL-1/NFkB signaling pathway in H292 cells infected with M. hyorhinis. This finding suggests that activation of IL-1/NFkB signaling pathway is the most likely mechanism of H292 cells resistance to Nutlin3.

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