The study of oxidative modification of proteins (OMP) can be performed based on the reaction with 2,4-dinitrophenylhydrazine, is a reagent for carbonyl compounds. The base model of the biological system (MBS) for relevant research can serve yolk lipoproteins in a spontaneous and Fe2+-induced oxidation. Another series of MBS were the bee products (propolis, homogenate drone brood, honey, royal jelly) as a test system, with antioxidant properties. MBS considered adding serum rats as biological environment, sensitive to changes in the conditions of free-radical processes.
Purpose – to justify the biological model system to test the method for assessing spontaneous and initiated by the oxidative modification of proteins in a pool of molecules of average weight.
Justification MBS to test method for assessing spontaneous and initiated by OMP in a pool of MSM conducted in the following series of observations: 1) in the MBS with the addition of yolk lipoprotein and without the addition of yolk lipoproteins in the spontaneous and Fe2+-induced oxidation, and 2) the IBS with and without the addition of serum the blood of rats with spontaneous and Fe2+-induced oxidation, and 3) the IBS with and without the addition of yolk lipoprotein and serum of rats with spontaneous and Fe2+-induced oxidation.
The biochemical characteristics of possible MBS through a comparative evaluation in terms of spontaneous and initiated by OMP in a pool of MSM to support the possible use of their complex. Is set at the analyzed model of biological systems to the conditions of Fe2+-induced oxidation, which showed changes in levels of analyzed tests, compared to spontaneous oxidation.
Burmistrov S.O., KHodov D.A., Porotov I.G. Okislitelnaja modifikacija belkov
syvorotki krovi cheloveka, metod ee opredelenija// Voprosy medicinskojj khimii.
1995. № 1. S. 24 – 26.